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Analytical Ultracentrifugation Membrane Confined Analytical Electrophoresis Complementary Analytical Techniques
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- Analytical ultracentrifugation (AUF)
- A primary method for determining molecular weight and molecular size. The
concentration dependence of the molecular weight can provide the same
thermodynamic information as light scattering. The rate of movement of
molecules provides hydrodynamic size information.
- Key Features
- Small concentrations can be used
- Small volumes (10-480 uL depending on experiment)
- Does not require a standard
- Absorbance, Interference, and fluorescence detection are all possible
- Capabilities
- Determination of sedimentation and diffusion coefficiants:
- These coefficients are based on fundamental first principle analysis,
meaning that the use of a standard is not required.
- Wide detection range:
- From the common absorbance system, to the sensitive interference system, to
the selective fluorescence system, chances are the molecule of interest can be
detected in whatever concentration needed. For more information on each
detection system, please contact us below.
- Determination of hydrodynamic size:
- Analytical Ultracentrifugation provides a method by which the hydrodynamic
size distribution of a solution can be determined. This is useful for initial
size determination and for monitoring changes due to intermolecular binding.
- Sedimentation Velocity Sample
Preparation
Sedimentation Equilibrium Sample Preparation
Choosing an Optical System:
Use Absorbance Optics if: Use Interference Optics if: Use Both To: - Need Selectivity
- Want Added Sensitivity
- Cannot Dialyze the sample
- Buffer Absorbs
- Sample Does Not
- Precision is Required
- Extinction Coefficient Varies
- Need to use Short Column Centerpieces for Small Amount of Sample g(s)
- Determine Extinction Coefficient
- Test for Sample Purity
- Extend Concentration Range
